{"id":12554,"date":"2021-08-26T06:13:30","date_gmt":"2021-08-26T06:13:30","guid":{"rendered":"https:\/\/watchdmovies.net\/?page_id=12554"},"modified":"2023-11-03T04:18:12","modified_gmt":"2023-11-03T04:18:12","slug":"recombinant-proteinase-k","status":"publish","type":"page","link":"https:\/\/watchdmovies.net\/recombinant-proteinase-k\/","title":{"rendered":"Recombinant Proteinase K"},"content":{"rendered":"\n<h1>\n\t\tRecombinant Proteinase K, PCR grade  \n\t<\/h1>\n\t\t\t\t<img decoding=\"async\" src=\"https:\/\/watchdmovies.net\/wp-content\/plugins\/bb-plugin\/img\/pixel.png\" alt=\"\" itemprop=\"image\" onerror=\"this.style.display='none'\">\n\t\n<p>EC 3.4.21.64 &#8211; CAS# 39450-01 &#8211; M.W. 29.3 kDA<\/p>\n\t\t<h2>\t\n\tRecombinant Proteinase K<\/h2>\t\t\n\t\t\t<p><strong>Recombinant Proteinase K, lyophilized powder&nbsp;<\/strong> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;<\/p>\n\n\n\n\n\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/watchdmovies.net\/wp-content\/uploads\/2020\/08\/proteinase-K.jpg\" alt=\"proteinase K\" itemprop=\"image\" height=\"600\" width=\"800\" title=\"proteinase K\" onerror=\"this.style.display='none'\">\n\t<noscript class=\"ninja-forms-noscript-message\">\n    Notice: JavaScript is required for this content.<\/noscript><div id=\"nf-form-5-cont\" class=\"nf-form-cont\" aria-live=\"polite\" aria-labelledby=\"nf-form-title-5\" aria-describedby=\"nf-form-errors-5\" role=\"form\">\n\n    <div   id="4vfzbgpkbc"    class=\"nf-loading-spinner\"><\/div>\n\n<\/div>\n        \n        <script>var formDisplay=1;var nfForms=nfForms||[];var form=[];form.id='5';form.settings={\"objectType\":\"Form Setting\",\"editActive\":true,\"title\":\"Request Sample or Quote for Proteinase K\",\"created_at\":\"2017-08-24 16:39:20\",\"form_title\":\"Contact 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       \n\t\t<h2><\/h2>\t\t\n\t\t\t<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/watchdmovies.net\/wp-content\/uploads\/2020\/08\/Proteinase-K-Recominant-PCR-grade-225x300.png\" alt=\"Proteinase K Recominant PCR grade\" width=\"176\" height=\"235\">Proteinase k from <em>Tritirachium Album &nbsp;Limber<\/em> and its recombinant variants are the most used enzyme for general digestion of proteins. It got its name from its unique ability to digest keratin, such as hair or nails. An essential lab reagent, proteinase K is a broad spectrum serine protease enzyme. It&#8217;s a member of the S8 (subcilisin) family of proteases. Proteinase K is also known under the names of protease K, endopeptidase<em> K<\/em>, and <em>Tritirachium <\/em>alkaline proteinase.<\/p>\n<p>Proteinase K digests native proteins by clearing peptide bonds adjacent to the caroboxyl group of aromatic and aliphatic amino acids with blocked alpha amino groups. It is activated by Ca++ ions, and it is stable in the presence of chelating agents.<\/p>\n<p>Proteinase K is commonly used for its broad specificity in sample digestion\/lysis. It&#8217;s main application is in isolation of high quality genomic DNA and RNA out of microbial and mammalian samples. During the process of tissue lysis or homogenization, proteinase K protects nucleic acids by rapidly degrading RNAses and DNAses enzymes, and as such is essential component of many molecular diagnostics, forensics and life sciences protocols and kits. &nbsp;To learn more about proteinase K we recommend you carefully read the <a href=\"https:\/\/en.wikipedia.org\/wiki\/Proteinase_K\">WikiPedia link<\/a> .<\/p>\n<p>Proteinase K is an essential reagent due to sudden increase in demand because of COVID-19 pandemic, this enzyme is in a short supply at the current market and has become a de-facto bottleneck for further proliferation of PCR tests. In order to break the testing bottleneck, Gojira Fine Chemicals LLC has bought to market the highly affordable, high quality, PCR grade proteinase K both in the form of lyophilized powder and in the form of a ready to use liquid buffered solution.<\/p>\n<p>Gojira Fine Chemicals Proteinase K is highly pure, PCR\/molecular biology grade recombinant enzyme, with guaranteed Proteinase K activity of &gt;30 U\/mg of powder and high specific enzyme activity of &gt;40U\/mg of protein. We recommend this enzyme to be stored at -20 deg. C for optimum shelf life. Gojira Fine Chemicals&#8217; Proteinase K enzyme is formulated to be highly stable over a wide pH range of 4- 12. It is a highly pure, sterile product, with no bio-bruden, and no presence of DNAse, RNAse, DNA and RNA contaminants. The recommended stock solution for Gojira Fine Chemicals Proteinase K is to dissolve 20-40mg\/mL of enzyme into an aqueous buffered solution of 50mM Tris-HCl (pH 7.5), 3mM CaCl2, with 50% Glycerol.<\/p>\n<p>This enzyme is readily available and we have it in stock in our Ohio, USA facility. We carry large inventory and are able to provide you at any time with multi kilogram shipment of this essential enzyme. We provide free samples for qualified institutions.&nbsp;<\/p>\n\t\t\t\t<h2><\/h2>\n\t\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t<li><\/li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/ul>\n\t\t\t<a href=\"https:\/\/watchdmovies.net\/wp-content\/uploads\/2019\/12\/PK1001-Proteinase-K-.pdf\" target=\"_blank\" role=\"button\" rel=\"noopener\">\n\t\t\t\t\t\tSpecifications\n\t\t\t\t\t<\/a>\n\t\t\t\t<br>\n\t\t\t\t<h2><\/h2>\n\t\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t<li><\/li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/ul>\n\t\t\t<a href=\"https:\/\/watchdmovies.net\/wp-content\/uploads\/2020\/08\/PK1001-Proteinase-K-0720001.pdf\" target=\"_blank\" role=\"button\" rel=\"noopener\">\n\t\t\t\t\t\tCOA\n\t\t\t\t\t<\/a>\n\t\t\t\t<br>\n\t\t\t\t<h2><\/h2>\n\t\t\t\t<ul>\n\t\t\t\t\t\t\t\t\t\t<li><\/li>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/ul>\n\t\t\t<a href=\"https:\/\/watchdmovies.net\/wp-content\/uploads\/2019\/12\/PK1001-Proteinase-K.pdf\" target=\"_blank\" role=\"button\" rel=\"noopener\">\n\t\t\t\t\t\tSDS\n\t\t\t\t\t<\/a>\n\t\t\t\t<br>\n\t\t<h2>\t\n\tProteinase K Recombinant, PCR Grade<\/h2>\t\t\n\t\t\t<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Catalog Number:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <\/strong>PK1001<\/p>\n<p><strong>Lot Number:<\/strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 0722005<\/p>\n<p><strong>CAS Number:&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; <\/strong>39450-01-6<\/p>\n<p><strong>Synonym(s):&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <\/strong>Peptidase K, Endoproteinase K, Endopeptidase K<\/p>\n<p><strong>EC Number:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <\/strong>3.4.21.64<\/p>\n<p><strong>Source:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <\/strong>Sequence of <em>Tritirachium. album<\/em>, purified from recombinant source<\/p>\n<p><strong>Molecular Weight:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <\/strong>29.3 kDa<\/p>\n<p><strong>Storage:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <\/strong>-20<sup>0<strong>&nbsp;<\/strong><\/sup>C<\/p>\n\n<table width=\"0\">\n<tbody>\n<tr>\n<td width=\"181\"><strong>Test<\/strong><\/td>\n<td width=\"312\"><strong>Specification<\/strong><\/td>\n<td width=\"186\"><\/td>\n<\/tr>\n<tr>\n<td width=\"181\"><strong>Appearance<\/strong><\/td>\n<td width=\"312\">White to off-white lyophilized powder<\/td>\n<td width=\"186\"><\/td>\n<\/tr>\n<tr>\n<td width=\"181\"><strong>Purity<\/strong><\/td>\n<td width=\"312\">\u2265 98%<\/td>\n<td width=\"186\"><\/td>\n<\/tr>\n<tr>\n<td width=\"181\"><strong>Activity<\/strong><\/td>\n<td width=\"312\">\u2265 30.0 U\/mg powder<\/td>\n<td width=\"186\"><\/td>\n<\/tr>\n<tr>\n<td width=\"181\"><strong>Specific Activity<\/strong><\/td>\n<td width=\"312\">\u2265 40.0 U\/mg protein<\/td>\n<td width=\"186\"><\/td>\n<\/tr>\n<tr>\n<td width=\"181\"><strong>DNase<\/strong><\/td>\n<td width=\"312\">Not detected<\/td>\n<td width=\"186\"><\/td>\n<\/tr>\n<tr>\n<td width=\"181\"><strong>RNase<\/strong><\/td>\n<td width=\"312\">Not detected<\/td>\n<td width=\"186\"><\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"679\"><strong>Application:&nbsp;<\/strong> Stock solution can be prepared as a 20-40mg\/mL solution in 50mM Tris-HCl (pH 7.5), 3mM CaCl<sub>2<\/sub>, 50% Glycerol.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Recombinant Proteinase K, PCR grade EC 3.4.21.64 &#8211; CAS# 39450-01 &#8211; M.W. 29.3 kDA Recombinant Proteinase K Recombinant Proteinase K, lyophilized powder&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp; &nbsp; &nbsp; Proteinase k from Tritirachium Album &nbsp;Limber and its recombinant variants are the most used enzyme for general digestion of proteins.&hellip;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"content-type":"","footnotes":""},"yoast_head":"\n<title>Recombinant Proteinase K - Gojira Fine Chemicals, LLC<\/title>\n<meta name=\"description\" content=\"This enzyme is readily available, and we have it in stock in our Ohio, USA, facility. Place your order now.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/watchdmovies.net\/recombinant-proteinase-k\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Recombinant Proteinase K - Gojira Fine Chemicals, LLC\" \/>\n<meta property=\"og:description\" content=\"This enzyme is readily available, and we have it in stock in our Ohio, USA, facility. 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